Microfluidics Core Facility Microfluidics Equipment
The following equipment can be used by the user to conduct the experiments in the Microfluidics Core Facility
Fully-featured microfluidic station
The microfluidic station consists of multiple instruments:
- High-Speed Camera (FastCam MiniUx50, Photron)
- Light microscope (AxioVert A1, Zeiss)
- Pressure-based Flow Controller (Elveflow)
- Syringe Pumps (ISPLab01,InfuseTek)
All instruments can be used after an introduction to the system. The microscope is also equipped with a LED and 3 different filter cubes (405nm, 488nm and 550nm) for observing fluorescently-labelled specimen. However, the high-speed camera is monochromatic, meaning that no fluorescent high-speed videos can be recorded. The frame rate of the high-speed camera is 2000fps at the lowest resolution of 1200x1048, by tuning the resolution, higher frames/second can be recorded. Given the final application either the syringe pumps or the pressure-based flow controller is used. Volumes from 0.5µl up to 150ml can be used with those syringe pumps and from flow rates starting at 0.002µl/min up to 165.8ml/min. In contrast, the Elveflow system works with pressures from 0 to 2bar, expanding the application range with the microfluidic set up in the facility. We also own a pressure sensor and a flow sensor which allow us to read out the proper pressure inside the channels and automatically calculates the flow rate from the applied pressure, respectively.
Micropuller (WPI, Pul-1000) and Microforge (WPI, MF200)
Besides PDMS-based microfluidic chips there are also glass capillary-nested devices. Mostly, such devices are sued to prepare water-in-oil, oil-in-water or water-in-oil-in-water droplets. To assemble such a device, it is necessary to cut and forge the opening of glass capillaries exactly to the size of the final droplet dimensions. To do so, we have a micropuller and a microforge, which can be used by the users after an introduction.